Rapid and simultaneous determination of 25-OH-vitamin D2 and D3 in human serum by LC/MS/MS: Validation and uncertainty assessment
نویسندگان
چکیده
25-OH Vitamin D3 and 25-OH Vitamin D2 were determined by rapid, simple and validated LC/MS/MS method without using SPE and isotopic standard. The relative standard deviations (RSD) were found to be 3.1 and 2.8 % for 25-OH Vitamin D3 and 25-OH Vitamin D2, respectively. The correlation coefficient was grater than 0.99 for both analytes in the calibration range. The recoveries at 100 μg L concentration averaged to 92 % for 25-OH vitamin D3 and 94 % for 25-OH-vitamin D2. The uncertainty of the measurements for 25-OH vitamin D3 and for 25-OH-vitamin D2 was 7.77 % and 7.24 %, respectively.
منابع مشابه
A fast and simple method for simultaneous measurements of 25(OH)D, 24,25(OH)2D and the Vitamin D Metabolite Ratio (VMR) in serum samples by LC-MS/MS.
BACKGROUND Rapid, easy and reliable measurement of the major vitamin D metabolites is required in order to fulfill the needs of a clinical routine laboratory. To overcome these challenges, we have developed and validated a LC-MS/MS method for the quantification of 25-hydroxyvitamin D2 and D3, epi-25-hydroxyvitamin D3 and 24,25-dihydroxyvitamin D3. METHODS Sample preparation was based on preci...
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